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Literature summary extracted from

  • Pollack, S.J.; Freeman, S.; Pompliano, D.L.; Knowles, F.R.
    Cloning, overexpression and mechanistic studies of carboxyphosphoenolpyruvate mutase from Streptomyces hygroscopicus (1992), Eur. J. Biochem., 209, 735-743.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.8.23 agriculture formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production Streptomyces hygroscopicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.23 gene cloned from a subgenomic library cloned by colony hybridisation in Escherichia coli AG1, subcloned into vector pET11a and overexpressed in Escherichia coli BL21(DE3) Streptomyces hygroscopicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.23 0.0022
-
 carboxyphosphinopyruvate pH 6.5, 30°C Streptomyces hygroscopicus
2.7.8.23 0.27
-
 carboxyphosphoenolpyruvate pH 6.5, 30°C Streptomyces hygroscopicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.23 Mg2+ reaction shows metal dependence Streptomyces hygroscopicus
2.7.8.23 Mn2+ increases activity, about 10times more active compared with the activity in presence of Mg2+ Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.23 carboxyphosphonoenolpyruvate Streptomyces hygroscopicus biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos (hydroxyphosphinyl)pyruvate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.23 no activity in Streptomyces hygroscopicus
-
-
-
2.7.8.23 Streptomyces hygroscopicus
-
 ATCC 21705
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.23
-
 Streptomyces hygroscopicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.23 additional information
-
 specific activity 37.6 U/mg x 1000 Streptomyces hygroscopicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.23 carboxyphosphinopyruvate
-
 Streptomyces hygroscopicus (hydroxyphosphinyl)pyruvate + CO2
-
 ?
2.7.8.23 carboxyphosphoenolpyruvate
-
 Streptomyces hygroscopicus ?
-
 ?
2.7.8.23 carboxyphosphonoenolpyruvate
-
 Streptomyces hygroscopicus (hydroxyphosphinyl)pyruvate + CO2
-
 ?
2.7.8.23 carboxyphosphonoenolpyruvate biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos Streptomyces hygroscopicus (hydroxyphosphinyl)pyruvate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.23 carboxyphosphoenolpyruvate mutase
-
 Streptomyces hygroscopicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.23 0.00076
-
 carboxyphosphinopyruvate pH 7.5, 30°C Streptomyces hygroscopicus
2.7.8.23 0.02
-
 carboxyphosphoenolpyruvate pH 7.5, 30°C Streptomyces hygroscopicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.8.23 5.5 8 stable in this range Streptomyces hygroscopicus